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Silver-stained 2-DE separation of proteins extracted from guinea pig otoconia. (A) Protein derived from 302 μg (dry weight) of otoconia from eight ears (equaling approximately 15 μg total protein) were extracted with EDTA. Gel was spiked with molecular mass markers (carbonic anhydrase, ovalbumin, and β-galactosidase: 29, 45, and 116 kDa, respectively; arrows). (B) Molecular mass markers only.
