Skip to main content
. 2007 Dec;13(12):2238–2251. doi: 10.1261/rna.610607

FIGURE 2.

FIGURE 2.

MBNL binds short CUG repeats with affinity similar to longer repeats, and the presence of mismatches is necessary for binding. The concentration of MBNL is shown above each lane in micromolar in the gel shift assay. (A) MBNL binding CUG90 repeats (lanes 1–6) with an apparent Kd of 230 nm. (B) MBNL binding to pyrimidine–pyrimidine mismatches. The sequence of the (CUG)4 RNA is shown below lanes 1–6. The boxed C-C (lanes 7–12) and U-C (lanes 13–18) represent the replacement of the U-U mismatches with these mismatches. (D) MBNL binding to purine–purine mismatches in place of the U-U mismatch; boxed sequence indicates mismatch replacement base pairs. (F) MBNL binding to G-U (lanes 1–6) and C-A (lanes 7–12) mismatches and Watson–Crick base pairs in place of the U-U mismatches. (H) Binding of MBNL to RNAs containing sequence alterations to the cytosine and guanine positions in CUG4 and a control RNA with no sequence similarity to CUG repeats. (C,E,G,I) Binding curves of the different mismatch and sequence alteration RNAs.