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Conditions of spontaneous dimerization in vitro of FL (7.7 kb), SD′ (3.8 kb), and 5′-Psi (878 nt) RNAs. Comparison between the ability to dimerize with (heat+D1) or without (D1) unfolding step. All experiments were performed with 1 pmol of RNA subjected to dimerization conditions as described in Materials and Methods, analyzed by native agarose gel electrophoresis, and visualized by ethidium bromide staining.
