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. 2007 Dec;13(12):2266–2276. doi: 10.1261/rna.713807

FIGURE 4.

FIGURE 4.

Analysis of heterodimerization between FL and SD′ RNAs. (A) FL and SD′ RNAs were cotranscribed by mixing the SD′ template cut either with BamHI, BstEII, or ClaI to, respectively, yield 1.8-kb, 2.6-kb, and 3.8-kb SD′ RNA (lanes 3,6,9, respectively) with the FL plasmid template. The cotranscribed RNA mixes were submitted to D1 conditions. Respective FL/SD′ heterodimers are indicated by a star and FL monomer and homodimer by open and black arrowheads, respectively. In parallel, each SD′ RNA was separately submitted to D1 dimerization conditions to form (open circles) SD′/SD′ homodimers (lanes 2,5,8). (Black circles) The different SD′ monomers were obtained by denaturation for 10 min at 85°C (lanes 1,4,7). (B) FL and SD′ RNAs form heterodimers through the Psi sequences. The same amount of FL/SD′ heterodimer (indicated by a star), previously formed by mixing SD′ and FL DNA templates, was incubated with increasing amounts of 5′-Psi (lanes 2–5) or 5′-ΔPsi (lanes 6–9) RNAs for competition experiments.