FIGURE 1.

Identification of suppressor(s) of ASF/SF2 depletion-induced HMW DNA fragmentation. (A) Strategy of isolating suppressor(s) of ASF/SF2 depletion-induced HMW DNA fragmentation. (B) ASF/SF2 depletion-induced HMW DNA fragmentation was partially suppressed in the selected stable transfectants. Selected colonies were expanded and grown for 48 h in the presence (+) or absence (−) of tet. Cell extracts were analyzed for the integrity of chromosomal DNA by 0.5% agarose (top panel) gel electrophoresis and for myc-tagged protein(s) by Western blot (bottom panel). The colony number is indicated.