Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Dec;13(12):2108–2115. doi: 10.1261/rna.734407

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007 RNA Society

PMC Copyright notice

FIGURE 3. — RNPS1 overexpression suppresses HMW DNA fragmentation, G2 cell cycle arrest, and hypermutation phenotypes in ASF/SF2-depleted cells. (A) Overexpression of RNPS1 reduces HMW DNA fragmentation in ASF/SF2-depleted cells. DT40-ASF and DT40-ASF/S1 cells were grown in the absence (−) or presence (+) of 1 μg/mL tet for the indicated times. The integrity of chromosomal DNA was analyzed by 0.5% agarose gel electrophoresis (upper panel) and pulse-field gel electrophoresis (PFEG) (lower panel). (B) Reversion of cell death in DT40-ASF cells and DT40-ASF/S1 cells after ASF/SF2 depletion. After a 2-d tet treatment, DT40-ASF and DT40-ASF/S1 cells were assayed for tet resistance, and the number of wells with surviving cells was determined. (C) Cell cycle phenotype of ASF/SF2-depleted cells. DT40-ASF and DT40-ASF/S1 cells, grown with or without 1 μg/mL tet for the indicated times, were stained with propidium iodide and analyzed by FACS. Raw data were quantitated and plotted, and the fraction of cells in G1, S, and G2/M phases are indicated.