TABLE 3.
Transformation of various strains of A. actinomycetemcomitans with genomic DNA.
|
Transformants/μg DNA1 |
|||||
|---|---|---|---|---|---|
| Strain | Colony Phenotype2 |
Serotype | Resident Plasmid |
katA3::IS903ϕkan3 | tadE::IS903ϕkan4 |
| DF2200N | R | a | pMB7 | 111.8 | 1.8 |
| Y4N | S | b | vector5 | 0 | 0 |
| Y4N | S | b | pMB7 | 5.9 | 0 |
| Aa307N | R | c | pMB7 | 7.6 | ND6 |
| IDH781N | R | d | pMB7 | 120.9 | 0.8 |
| IDH781N (Single Cells) |
R | d | pMB7 | 2573.3 | ND |
| IDH1705N | R | e | pMB7 | 23.8 | 0 |
| IDH1705N | S7 | e | pMB7 | 0.16 | ND |
| CU1000N8 | R | f | pMB7 | 0 | 0 |
| CU1060N8 | S | f | pMB7 | 0 | 0 |
| NJ9100N | R | f | pMB7 | 0 | ND |
1
Transformation was done as described in Materials and Methods (Section 2.4). Transformants were selected on AAGM plates containing kanamycin.
2
S: smooth; R: rough.
3
Genomic DNA from Y4N katA3::IS903ϕkan.
4
Genomic DNA from CU1000N tadE::IS903ϕkan.
5
Vector (pJAK17) control for all other strains gave no transformants.
6
ND, not determined.
7
A spontaneous smooth variant of IDH1705N with the formal name MB1221.
8
The same results were obtained with pMB8 as the resident plasmid.