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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Gene. 2007 May 1;399(1):53–64. doi: 10.1016/j.gene.2007.04.026

TABLE 4.

Allelic exchange after transformation with IncQ Plasmid DNA.

Strain1 Colony
Phenotype2 		Transforming
DNA3 		Relevant
Features		 Transformants4
/μg DNA		 Catalase
Phenotype5
DF2200N R pJAK13 Vector, circular 0 ND
DF2200N R pMB40 2 USS, circular 1.7 × 104 +
DF2200N R pMB22 katA3::IS903ϕkan
3 USS, circular		 5.1 × 102 +
DF2200N R pMB22 + PstI katA3::IS903ϕkan
3 USS, linear		 1.7 × 102 −
Y4N S pJAK13 Vector, circular 0 ND
Y4N S pMB22 katA3::IS903ϕkan
3 USS, circular		 3.0 +
Y4N S pMB22 + PstI katA3::IS903ϕkan
3 USS, linear		 6.0 −
1

Both strains contained the plasmid pMB7 and were transformed as described in Materials & Methods (Section 2.4).

2

S, smooth; R, rough

3

0.5 μg plasmid DNA was used for each transformation.

4

Transformants were selected on AAGM plates containing kanamycin for pMB22 or streptomycin for pJAK13 and pMB40.

5

+ indicates that all transformants tested showed catalase activity; − indicates that all transformants tested did not show catalase activity; ND, not done. All Y4N transformants were tested; 30 DF2200N transformants were tested for each different DNA. Allelic exchange was confirmed by PCR (see text).