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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Gene. 2007 May 1;399(1):53–64. doi: 10.1016/j.gene.2007.04.026

TABLE 5.

Transformation with a suicide vector.1

Transforming DNA2 Relevant Features Transformants/μg DNA
pUC19 Vector
circular plasmid DNA		 0
pMB78 2 USS
circular plasmid DNA		 0
pMB79 katA3::IS903ϕkan, 2 USS,
circular plasmid DNA		 2.9 × 104
pMB79 + SpeI katA3::IS903ϕkan, 2 USS,
linear plasmid DNA		 1.1 × 105
pMB79 + KpnI katA3::IS903ϕkan, 2 USS,
linear plasmid DNA		 2.3 × 105
Y4N katA3::IS903ϕkan katA3::IS903ϕkan genomic DNA 5.0 × 102
1

DF2200N(pMB7) was transformed as described in Materials and Methods (Section 2.4). Transformants were selected on AAGM plates containing kanamycin.

2

0.1 μg DNA for plasmids; 20 μg DNA for genomic DNA.