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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Gene. 2007 May 1;399(1):53–64. doi: 10.1016/j.gene.2007.04.026

TABLE 6.

Homology sufficient at one end for allelic exchange.

Plasmid Homology (bp)
beyond the two ends
of the transposon1 		Relative
orientation2 		Transformants3/μg DNA
Linear DNA
 Circular DNA
KpnI cut SpeI cut
pMB89 1318, 78 + 1.3 × 105 6.6 × 104 3.0 × 104
pMB90 170, 1226 − 2.2 × 103 3.2 × 103 5.0 × 102
pMB91 288, 1108 + 6.2 × 104 1.2 × 105 4.0 × 104
pMB92 481, 915 − 5.7 × 103 1.5 × 103 5.0 × 102
pMB93 275, 1121 − 9.0 × 102 7.0 × 102 1.0 × 103
1

Homology beyond the two ends of the transposon was determined by sequencing outwards from the ends of the transposon.

2

+ indicates katA and neo are oriented in the same direction; − indicates they are oriented in opposite directions. The orientations were determined by sequencing outwards from either end of the transposon.

3

DF2200N(pMB7) was transformed as described in Materials and Methods (Section 2.4). All transformants tested had replaced the wild type katA gene (chromosomal) with a mutant gene (plasmid-borne), as determined by PCR.