Skip to main content
. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Hear Res. 2007 Jul 19;233(1-2):46–53. doi: 10.1016/j.heares.2007.07.003

Figure 4.

Figure 4

Timelapse microscopy of hair cells from single neuromast (OC1) in a living zebrafish exposed to neomycin. Hair cell nuclei were prelabeled with YO-PRO1, exposed to 200 μM neomycin, and imaged at the labeled timepoints. The first timepoint measured was at 5 minutes as this was the time required to anesthetize and prepare the fish for timelapse imaging. Note that as early as five minutes there was already evidence of hair cell damage. Overall, morphologic changes of hair cell death after neomycin treatment were seen much more rapidly than were seen during cisplatin exposure in Figure 3. Triangles indicate fragmented nuclei. Arrows indicate condensed nuclei. Scale bar = 10 μm.