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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Feb 13;1771(4):514–521. doi: 10.1016/j.bbalip.2007.02.002

Fig. 3. The effect of inhibitors on the increase in nuclear PLC‐activity at 30 min (A) and 11 h (B) after addition of FBS.

Fig. 3.

Exponentially growing HL‐60 cells were washed and released into serum‐free medium. After 24 h, cells were stimulated with 10% FBS (v/v.). PI‐PLC inhibitor ET‐18‐OCH3 (10 µM), MEK inhibitor PD 98059 (20 µM) or PI3K‐inhibitor LY 294002 (25 µM) were added either immediately (A) or 6 h after addition of FBS (B). Nuclei were isolated from cells harvested at 30 min and 11 h after addition of FBS, and PLC‐activity was assayed using PtdIns(4,5)P2 as substrate. Results are means ± S.E.M. for three different experiments, each performed in duplicate. *P < 0.05 (Student’s t‐test) with respect to the control.