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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: J Immunol Methods. 2007 Aug 9;326(1-2):127–138. doi: 10.1016/j.jim.2007.07.016

Figure 4. Comparison of treatments and recovery methods for sorted antigen-specific CD8+ T-cells.

Figure 4

Groups are separated by treatment without (no fixation) and with (fixation) buffered formaldehyde. The Morisita-Horn similarity index (C-MH: S.I.) was used to assess the similarity and the associated p-value indicates whether two samples are significantly different. The frequencies (%) and complementarity-determining region 3 (CDR3) amino acid sequence identities of recovered TRB gene rearrangements are shown for each condition. Phenol/guanine isothiocyanate was used to isolate template in (A); location and FACS sorting strategies are compared in (B); and oligo-dT bead-based mRNA recovery data from sorted CD8+ T-cell populations specific for antigens derived from HIV and CMV are shown in (C) and (D) respectively.