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Chemical shift changes of backbone amides of the wild-type and mutated Tom20s upon binding to the pALDH presequence peptide at the molar ratio of 1:1. The chemical shift change of each backbone amide in [1H, 15N]HSQC spectra was calculated according to the equation, [Δδ(1H)2+(Δδ(15N)/5)2]1/2. The positions of proline residues are indicated with the letter ‘p' in the top panel.
