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Arc-like filaments persist in the presence of A22. Low magnification cryo-EM views (A, C) and corresponding segmented 3-D reconstructions (B, D) of a NA1000 cell (A, B) and a cell overexpressing FtsZG109S (C, D), both after treatment with A22, again showing arc-like filaments. Scale bar in (A) 500 nm. For scale in (C), the circular hole in the carbon support film on the EM grid has a diameter of 1.3 μm. See also supporting fLM studies in Supplementary Figure S10.
