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. 2007 Nov 15;26(24):4996–5006. doi: 10.1038/sj.emboj.7601921

Figure 4.

Figure 4

IGS1-R is a 3′-heterogeneous transcript polyadenylated by Pap1. (A) Primer extension analysis of IGS1-R performed on poly(A)+ RNA from oligonucleotide NTS1 F3 (see C). (B) Ethidium-stained 6% acrylamide gel of 35-cycle 3′ RACE reactions using NTS1 R2 (see C). (C) Schematic of IGS1-R showing mapped 3′ ends and primer locations for 3′ RACE and primer extension. (D) Northern analysis of TRAMP and Pap1 mutants grown at 25°C in YPD and shifted to 37°C for 1 h, probed with NTS1 HpaI and SCR1. (E) trf4Δ strains were transformed with Trf4 plasmids (Vanacova et al, 2005), grown to mid-log in synthetic media and probed with NTS1 HpaI and ACT1. Error bars=±1 standard error, n=3.