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. 2007 Nov 15;26(24):4996–5006. doi: 10.1038/sj.emboj.7601921

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Copyright © 2007, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.

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The role of Trf4 in rDNA recombination. (A) Pulsed field analysis of trf4Δ top1Δ strains with Trf4 plasmids. One TOP1 and two top1Δ samples are shown in each case. Cells were grown in synthetic media and harvested at stationary phase approximately 50 generations after TOP1 deletion. Gel was run and probed as in Figure 5B. (B) An rDNA MET25 reporter was randomly integrated in each strain, and cells were grown to mid-log before plating on modified lead acetate plates at 25°C. Once colonies were ∼1 mm diameter, they were imaged and scored for the presence or absence of sectoring. (C) Recombination frequency assessed by colony sectoring. Three separate MET25 insertion clones were analyzed twice for each strain. Error bars=±1 standard error, n=6.