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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Arch Biochem Biophys. 2007 Mar 8;463(1):19–26. doi: 10.1016/j.abb.2007.02.016

FIG. 1. Coq9p is peripherally associated to the inner mitochondrial membrane, facing toward the matrix side.

FIG. 1

A) Mitochondria were subjected to hypotonic swelling and centrifugation to separate intermembrane space protein (IMS) and mitoplasts. The mitoplasts were either sonicated or treated with 0.1 M Na2CO3, pH 11.5, then separated via 100,000 × g centrifugation into pellet (P) or supernatant (S) fractions. B) Intact mitochondria or mitoplasts were treated with 100 μg/ml Proteinase K for 30 min on ice, in the presence or absence of detergent. Equal aliquots of pellets and TCA-precipitated soluble fractions were analyzed. Control polypeptide markers: (Atp2) β subunit of F1 ATPase, peripheral inner membrane; (Cyt b2) cytochrome b2, inter-membrane space; (Cyt c1) cytochrome c1, integral inner membrane; and (Hsp60), soluble matrix.