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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Arch Biochem Biophys. 2007 Mar 8;463(1):19–26. doi: 10.1016/j.abb.2007.02.016

FIG. 2. Expression of ABC1/COQ8 on low- or multi-copy plasmids suppress yeast coq9-1 mutants.

FIG. 2

A) The coq9-1 mutant, C92/UL3, is rescued for growth on the nonfermentable carbon source, glycerol, when transformed with ABC1/COQ8. The plasmids used were vector only (pRS426) or contained one of the following genes: ABC1/COQ8 (p3HN4, p4HN4 and T3) or COQ9 (T2). All plasmids are present in multicopy, except p3HN4, a low copy plasmid. B) Whole cell lysates of yeast (corresponding to 0.2 OD600nm) were analyzed by immunoblot with antisera against the indicated polypeptides. Cell lysates were prepared from W303-1A (W), W303ΔCOQ9 (Δ9) and the coq9-1mutant (C92/UL3) harboring the designated plasmids.