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. Author manuscript; available in PMC: 2007 Nov 19.
Published in final edited form as: Protein Expr Purif. 2006 Sep 15;52(2):273–279. doi: 10.1016/j.pep.2006.09.004

Figure 2.

Figure 2

Dependence of PDR expression in C41 E. coli on the OD of cell culture at 600 nm at the time of induction with IPTG. Temperature before induction 37 °C ( ■ ) and 30 °C ( ▲ ). Concentration of PDR in cell culture was measured as described in Materials and Methods. Test growths in 50 mL TB media in 0.25 L conical flasks in the presence of 0.2 mg/mL Fe citrate, 2 mM cysteine, 0.2 mg/mL FeSO4 and 100 mg/L carbenicillin at 30 °C, 200 rpm for 40 hours after induction.