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. Author manuscript; available in PMC: 2007 Nov 19.
Published in final edited form as: Protein Expr Purif. 2006 Sep 15;52(2):273–279. doi: 10.1016/j.pep.2006.09.004

Figure 3.

Figure 3

SDS PAGE of lysed cultures of C41 E. coli expressing PDR. 1. Protein ladder (5 μL, BioRad Broadband); 2. Cells harvested from the media at pH 8.5-8.8; 3. Cells harvested when the pH of the media was less that 7.7. For lanes 2 and 3, 0.2 μL of lysed cell culture (after 1 minute sonication of freeze-thawed aliquote) were applied. Recombinant protein expression was performed using the optimized protocol as described in the text.