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. Author manuscript; available in PMC: 2007 Nov 19.
Published in final edited form as: Protein Expr Purif. 2006 Sep 15;52(2):273–279. doi: 10.1016/j.pep.2006.09.004

Table 1.

Additions affecting PDR yield1 P-value Increase in PDR
expression yield ,
% ± SE
A 1 Any Fe containing compound2 3.2 10−5 83 ± 19
2 Any Sulfur containing compound3 0.79 − 6 ± 23
B 1 Cysteine 0.63 16 ± 33
2 Ferrous Sulfate 0.17 44 ± 37
3 Ferric Citrate 9.6 10−5 130 ± 32
4 Ferric Ammonium Citrate 1.6 10−2 85 ± 35
C 1 Ferric Citrate 1.3 10−3 92 ± 28
2 Ferric Ammonium Citrate 2.7 10−2 57 ± 25
3 Cysteine (if FC or FAC is also present ) 0.71 11 ± 28
D 1 Ferric Citrate 3.6 10−3 78 ± 26
2 Ferric Ammonium Citrate 8.2 10−2 47 ± 26
3 Ferrous Sulfate ( if FC or FAC is also present ) 3.2 10−2 60 ± 28
1

See Materials and Methods for concentrations.

2

Including Ferrous Sulfate, FC and FAC

3

Including Cysteine and Ferrous Sulfate