Skip to main content
. Author manuscript; available in PMC: 2007 Nov 19.
Published in final edited form as: Insect Biochem Mol Biol. 2005 Nov 8;36(1):37–46. doi: 10.1016/j.ibmb.2005.10.004

Fig. 1.

Fig. 1.

Immune-induced cleavage of Nec occurs at the N-terminus. (A) Schematic representation of the Nec-Tag transgene. The Myc tag is N-terminal, between the signal peptide (SP) and the polyglutamine rich N-terminal extension (XQXQQ). The Ha tag is at the C-terminal of the serpin molecule. (B) Western blot of hemolymph from wild type (WT) and Nec-tag (Df(2R)pk-78k/nec²; P[da-Gal4]/P[UAS-nectag]) flies. Samples were taken from unchallenged (0 h) flies or 6 h after challenge with Micrococcus luteus (6 h). The same membrane was used for probing with anti-GST-Nec, anti-Ha and anti-Myc antibodies.