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RNA thermal denaturation profiles for SIV17−50. (A) First derivative plot of change in UV absorbance vs. temperature (red), with fitted curves showing the deconvoluted transitions as dashed lines. The Tm values are shown above each peak. (B) First derivative plot of change in UV absorbance vs. temperature of SIV17−50 (red) compared to loop sequence mutants G31C (green), G36C (blue) and G31C, G36C (magenta).
