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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Exp Cell Res. 2007 Jul 28;313(16):3645–3657. doi: 10.1016/j.yexcr.2007.07.023

Fig. 2. Time course for microvessel disruption induced by endothelial cell apoptosis.

Fig. 2

Photomicrographs (200x) of representative fields of capillary sprouts developed by HDMEC-LXSN (A, D and G) or HDMEC-iCaspase-9 cells (B, E and H) in type I collagen matrices. Cells were cultured for 4 days in endothelial growth medium supplemented with 50 ng/ml VEGF, followed by 7 days culture in medium supplemented with 50 ng/ml VEGF165 alone (A-C), 50 ng/ml VEGF165 + 100 nM AP20187 (D-F), or 100 nM AP20187 alone (G-I). Arrowheads point to capillary sprouts. (C, F and I) Graphs depict the number of sprouts per microscopic field (100x). Horizontal bars depict the duration of treatment with 50 ng/ml VEGF165. (F and I) Black arrows indicate the beginning of treatment with AP20187. At daily intervals, the number of sprouts was counted in 6 random fields from triplicate wells per condition, from three independent experiments. * P<0.05.