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. 2007 Nov 19;179(4):627–633. doi: 10.1083/jcb.200705062

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Interaction of M protein with LUVs. (A) M protein adsorption on PC–PE–cholesterol LUVs at different protein/lipid ratios measured by gradient flotation. The same protein concentration was loaded for all bands. The control fraction (M, no lipids) was taken at the same level as the liposome fraction. Positive control shows the M protein band. (B) Sequential additions of 0.3 μM of M protein or BSA to LUV caused dequenching of Rh-DOPE or BODIPY-G_m1 fluorescence (red and black circles). No changes were detected for nonquenched dyes (red and black diamonds) or when BSA was added (dark yellow circles). (E) The same additions of M protein induce the release of LUV-entrapped ANTS/DPX (blue squares) or 70-kD FITC-conjugated dextrans (green squares), seen as changes of normalized fluorescence intensity. The addition of the same amount of the protein mixed with α-chymotrypsin 1:5 causes minor release of ANTS/DPX and dextrans (blue and green triangles). Bars show SD.