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. 2007 Nov 19;179(4):701–715. doi: 10.1083/jcb.200703040

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Nbk expression induces caspase processing and apoptotic DNA fragmentation in HCT116 wt and HT116 Bak-deficient cells but not in Bax knockout cells. (A) Bax and Bak expression levels in different HCT116 cell lines. (B) Western blot analysis for Nbk expression and caspase 9 and 3 cleavage. HCT116 cells were infected with the recombinant adenovirus Ad-mycNbk-tTA and cultured for 24 h in the presence (off) or absence (on) of doxycycline (Dox). Control cells were mock treated and grown in the absence of doxycycline. *, unspecific band. (C) Flow cytometric measurement of DNA content. Cells were treated as described in B. Cells displaying a sub-G1 hypodiploid DNA content are considered apoptotic. Data are means ± SD from three independent experiments.