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. 2007 Nov 19;179(4):701–715. doi: 10.1083/jcb.200703040

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Nbk expression induces oligomerization and clustering of EGFP-Bax but not -Bak. (A) DU145 cells stably expressing EGFP-Bax or -Bak were transduced with Ad-mycNbk-tTA and cultured under off or on conditions. 24 h after infection of the cells, images were acquired by the use of a fluorescent microscope. Bar, 50 μm. (B) Subcellular localization pattern of EGFP-Bax. Mitochondria of DU145 EGFP-Bax cells were stained red by the use of an antibody against the Tom 20 protein and counterstained for DNA with DAPI. Bax shows a diffuse pattern of localization in nonapoptotic cells, concordant with its preferentially cytoplasmic localization (top). After Nbk expression Bax coalesces into large clusters adjacent to mitochondria (bottom). Bar, 25 μm.