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. 2007 Nov 19;179(4):747–760. doi: 10.1083/jcb.200706090

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Normal skin morphogenesis but enhanced UV susceptibility in Prdx6 knockout mice. (A) Back skin lysates (30 μg protein) from homozygous (−/−) and heterozygous (+/−) Prdx6 knockout mice and wild-type controls (+/+) were analyzed by Western blotting for the presence of Prdx6 and β-actin. (B) Paraffin sections (6 μm) were stained using the Masson trichrome procedure. Bars indicate 100 μm. D: dermis; E: epidermis; HF: hair follicle. The basal cell layer of the epidermis is indicated with arrowheads. (C) Mice (n = 10 per genotype) were irradiated with 60 J/cm² UVA. Paraffin sections from the back skin of the irradiated mice were stained with H/E. Bars indicate 20 μm. Apoptotic sunburn cells in the epidermis (arrowheads) were counted. (D) Sections from UVA-irradiated mice (n = 10 per genotype) were stained with an antibody to p53, and p53-positive cells in the epidermis were counted. (E) Mice (n = 5 per genotype) were irradiated with 100 mJ/cm² UVB. Frozen sections from the back skin of the irradiated mice were analyzed by immunofluorescence using an antibody against cleaved caspase-3. Bars indicate 20 μm. Apoptotic cells in the epidermis were counted. Statistical analysis was performed using Mann-Whitney-U test for non-Gaussian distribution. *, P = 0.0146; **, P = 0.0068 (UVA irradiation) and *, P = 0.015 (UVB irradiation).