Figure 6.

Enhanced oxidative stress in 5-d wounds of Prdx6 knockout mice. (A) Lysates of 5-d wounds from female and male mice (female 18.3 μg and 10 μg, male 20 μg of total protein) were analyzed for the presence of oxidized proteins by oxyblot analysis. The membrane was reprobed with an antibody to β-actin. (B) 12 wound halves of 9 female wild-type mice and 12 wound halves of 9 female knockout mice were analyzed by immunofluorescence for the presence of nitrotyrosine positive cells. The percentage of nitrotyrosine positive cells was determined using the ImageJ program. Statistical analysis was performed using the Mann-Whitney-U test. *, P = 0.0464. Representative stainings are shown in C. Nitrotyrosine-positive cells are indicated by arrowheads. Nuclei were counterstained with Hoechst. Bars indicate 50 μm. G: Granulation tissue, HE: Hyperproliferative epithelium.