Figure 2.

Sequence of DGKζ cDNAs isolated from skeletal muscle. A skeletal muscle library was screened with a probe from the endothelial cell DGKζ cDNA, and novel 4.1- and 3.4-kb cDNAs were isolated and sequenced. The 4.1-kb clone had a novel 5′ sequence, as compared with the endothelial cell clone, but was identical after position 853. The 3.4-kb clone was the same as that from endothelial cells except for a 77-bp deletion. (A) The unique nucleic acid sequence and deduced amino acid sequence of the 4.1-kb skeletal muscle clone. (B) A comparison between the putative translation initiation site of the 4.1-kb clone and the Kozak consensus sequence. (C) The deleted nucleotide sequence and corresponding amino acid sequence of the 3.4-kb clone.