Figure 2.
Binding of the maltose-binding protein fusions (MBP)-C7-C7 and MBP-Sp1C-C7 with duplex DNA oligonucleotides containing various target sequences. (A) MBP-C7-C7 protein was used to shift the double-stranded DNA probes containing the target sequences listed on top of each panel [from left to right: C7-C7 site, Sp1C-C7 site, C7 site, and (GCG)6 site]. The protein concentration is given in nanomoles (nM) beneath each lane with a 2-fold serial dilution from left to right in each panel. (B) MBP-SP1C-C7 protein was titrated into gel shift reactions with probes containing target sequences (from left to right: Sp1C-C7 site, C7-C7 site, C7 site, and Sp1C site) as listed on top of each panel. The protein concentration is labeled in nM beneath each lane, with a 2-fold serial dilution from left to right in each panel.