Figure 4.

Transcriptional regulation mediated by six-finger proteins in living cells. (A) HeLa cells were transiently transfected in triplicate with 2.5 μg of the indicated reporter plasmids and 2.5 μg C7-C7-VP16 expression plasmid. Luciferase activities were measured 48 hr later and normalized to the control β-galactosidase activity. The relative light units are given on top of each column (error bar = SD). (B) HeLa cells were transfected with 2.5 μg of the indicated reporter plasmids and either no C7-C7-KRAB expression or 1 μg of the C7-C7-KRAB expression plasmid by using LipofectAmine (GIBCO/BRL) as the transfection reagent. Luciferase activities were measured 48 hr later, and normalized to the control β-galactosidase activity. The relative light unit values were labeled on top of each column (error bar = SD).