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. Author manuscript; available in PMC: 2007 Nov 19.
Published in final edited form as: J Biol Chem. 2007 Apr 26;282(24):17613–17622. doi: 10.1074/jbc.M702799200

FIGURE 4. Dependence of protein kinase C activity on the concentrations of ATP and E. coli-expressed human CTP synthetase 1.

FIGURE 4

A, mammalian protein kinase C (0.5 unit/ml) was incubated with human CTP synthetase 1 (50 µg/ml) and the indicated concentrations of [γ-32P]ATP (11,000 cpm/pmol) for 10 min. B, mammalian protein kinase C (0.5 unit/ml) was incubated with 50 µM [γ-32P]ATP (11,000 cpm/pmol) and the indicated concentrations of human CTP synthetase 1 for 10 min. After the phosphorylation reactions, the samples were subjected to SDS-PAGE. The SDS-polyacrylamide gels were dried and the phosphorylated proteins were subjected to phosphorimaging analysis. The relative amounts of phosphate incorporated into CTP synthetase 1 were quantified using ImageQuant software. Each data point represents the average of duplicate determinations.