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. Author manuscript; available in PMC: 2007 Nov 19.
Published in final edited form as: J Biol Chem. 2007 Apr 26;282(24):17613–17622. doi: 10.1074/jbc.M702799200

FIGURE 5. Effect of protein kinase C phosphorylation on E. coli-expressed human CTP synthetase 1 activity.

FIGURE 5

Human CTP synthetase 1 (160 µg/ml) was phosphorylated with mammalian protein kinase C (0.5 unit/ml) for the indicated time intervals using unlabeled ATP. Following the phosphorylations, samples were diluted 4-fold into the standard CTP synthetase 1 reaction mixture and activity was measured by following the conversion of UTP to CTP at 291 nm using a recording spectrophotometer. Each data point represents the average of triplicate determinations ± S.D.