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. Author manuscript; available in PMC: 2007 Nov 19.
Published in final edited form as: J Biol Chem. 2007 Apr 26;282(24):17613–17622. doi: 10.1074/jbc.M702799200

Table 2.

Plasmids used in this work

Plasmid Relevant characteristics Source or Ref.
YEpLac181 Multicopy E. coli/yeast shuttle vector with LEU2 (76)
YEpLac195 Multicopy E. coli/yeast shuttle vector with URA3 (76)
pDO105 Derivative of YEpLac181 with the ADH1 promoter and multiple cloning sites (8)
pDO134 URA7 derivative of YEpLac195 (8)
pDO105-hCTPS1 CTPS1 derivative of pDO105 (52)
pDO105-hCTPS1(T455A) CTPS1T455A derivative of pDO105-hCTPS1 (53)
pDO105-hCTPS1(S462A) CTPS1S462A derivative of pDO105-hCTPS1 This work
pDO105-hCTPS1(S462A,T455A) CTPS1S462A,T455A derivative of pDO105-hCTPS1(T455A) This work
pBM743 Single copy vector containing the GAL1 promoter with URA3 (77)
pGAL1::PKC1(R398A::HA) PKC1(R398A::HA) derivative of pBM743 (61)