Figure 3.

Immunoblot quantitation of GPx in Trsp (+/−) mice. The band for GPx of the relative molecular mass ≈26 kDa is seen between the molecular weight markers of 30 kDa and 21.5 kDa. Two independent antibodies against GPx were used. (A) Sheep anti-bovine GPx. (B) Rabbit anti-bovine GPx. Lanes 1, 2, 5, and 6 show liver extracts from the Trsp (+/+) mice, whereas lanes 3, 4, 7, and 8 show those from Trsp (+/−) mice. On lanes 9 and 10, purified human GPx was loaded at 1 μg and 10 μg, respectively. The results of the densitometric determination of the bands are summarized in Table 3.