Table 3.
Relative amounts of GPx in Trsp (+/−) mice
Organ | mRNA, % | Protein, % | Enzyme activity, % |
---|---|---|---|
Liver | 84 | 104 | 97 |
Kidney | 131 | ND | ND |
Relative amounts of GPx mRNA in the Trsp (+/−) mice are shown as percentages to the Trsp (+/+) mice, both calibrated to the amounts of G3PDH mRNA, whereas those of GPx protein are shown as the mean ratio of the GPx western band intensities shown in Fig. 3. The GPx enzyme activity is shown as the ratio of the Trsp (+/−) mouse liver S-100 fraction activity per milligram of protein compared with that in the Trsp (+/+) liver. The mean GPx activity ±SE was 79 ± 4 units per mg for the Trsp (+/+) liver and 71 ± 4 units per mg for the Trsp (+/−) liver. ND, not determined.