Figure 3.
Stability of cII in λ-infected cells. Cells of AD16 (wild-type; •), AK990 (ΔhflK-hflC; ▪), AK525 (zgj-525::IS1A; ▴), AD16/pBAD18 (vector; ○), and AD16/pKH178 (para-hflKC; ◊) were grown at 37°C (A) or at 30°C (B) to a mid-logarithmic phase. To the latter two strains, 0.4% arabinose was added 1 hr before UV irradiation. Cells were UV-irradiated and infected with λ. After 5 min (37°C) or 8 min (30°C), cells were pulse-labeled for 1 min with [35S]methionine and chased as indicated. Total labeled proteins were precipitated by trichloroacetic acid and separated by SDS/PAGE. Radioactivities associated with the cII band were quantitated, and values relative to those without chase are presented.