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β-Galactosidase-specific activities in cells harboring the GAL4/MAC1 fusion gene (top construct) to mutant fusion genes. ∗, The presence of the MAC1up1 mutation resulting in the His → Gln substitution (20). The numbers over the Mac1 portion of the fusion indicate the Mac1 residues encoded by the fusion genes. One internal deletion (Δ252–341) was constructed in which residues 252–341 were deleted. The results are the mean (± SEM) of three independent measurements.
