Figure 2.

(a) CD curves of N5-C85 alone (×); difference CD curves (complex-free DNA) of N5-C85 with the Skn recognition duplex (+), and of N5-C85 with the nonspecific control duplex (⋄); ○, difference between the complex and the sum of the spectra of the free protein and the free SKN18 duplex. (b) Thermal denaturation curves of N5-C85 (curve a, +), N5-C77 (curve b, ○), and N5-C74 (curve c, □) in the presence of SKN18; N5-C85 in the presence of NS18 (curve d, +); and N5-C74 in the presence of NS18 (curve e, □). Although N5-C74 has a lower melting temperature and a slightly broader transition than the other proteins, a comparison of curves c and e confirms that it binds specifically to the SKN18 site.