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UV/vis spectrum of apo (solid line) and reconstituted (dashed line) {Fe3+}Trx-SOD. The spectra of Trx-SOD (1.1 × 10−4 M) in 20 mM Hepes (pH 8.0)/25 mM NaCl at 21°C, before and after iron reconstitution with ferrous ammonium sulfate in air are shown.
