Figure 2.

Indirect immunofluorescence of FRTL-5 and FRT cells using anti-NIS Ab. FRTL-5 and FRT cells were cultured as described previously (10). Cells were trypsinized, harvested, and seeded onto poly-(lysine)-coated coverslips (≈50% confluence). Next day cells were fixed in 4% paraformaldehyde and incubated either in the presence of anti-NIS Ab (1:500 B, D, F, J) or pre-immune (H) or in the presence (D, F, H, J) or absence (B) of methanol to expose intracellular antigen. Cells in F were incubated with 20 μg of peptide. (Left) Phase contrast images (A, C, E, G, and I) of Right side (B, D, F, H, and J). Cells were visualized as described in Materials and Methods.