Figure 5.

In vivo regulation of NIS. Thyroidectomies were performed on control, experimental, or hypophysectomized female Fisher rats (Charles River Breeding Laboratories). Hypophysectomy was carried out 10–20 days prior to thyroidectomy. Thyroid membrane fractions were subjected to immunoblot analysis with anti-NIS Ab as described in Fig. 1. Thyroid membrane fractions (140 μg of protein) from control rats, lane 1; PTU-treated rats, lane 2; iodide-deficient rats, lane 3; hypophysectomized rats (HPX), lane 4; hypophysectomized rats that received bovine TSH (1 international unit, Calbiochem) administered intraperitoneally 24 hr prior to thyroidectomy (HPX + TSH), lane 5; FRTL-5 membranes (40 μg of protein), lane 6.