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. 2007 Dec 1;21(23):3110–3122. doi: 10.1101/gad.1594007

Figure 4.

Figure 4.

Dnmt3b1 overexpression increases the size of colonic microadenomas, increases expression of Igf2, and decreases expression of Sfrp2, Sfrp4, and Sfrp5 in colon tumors. (A) Horizontal en face sections of colon mucosa derived from a control and Dnmt3b1-expressing mouse (H&E stain, 10×). (Arrows) Microadenomas; (LF) lymph follicule. The size of microadenomas in the mouse with increased Dnmt3b1 expression was much larger than in the control mouse. (B) The average size of colonic microadenomas in Dnmt3b1-expressing mice was increased 1.7-fold when compared with controls (364 μm ± 38 vs. 211 μm ± 25 colon; P < 0.0005). Values represent mean ± SE. (C) Quantitation of Igf2 expression in colon tumors normalized to β-actin expression using real-time PCR. The relative Igf2 expression in tumors derived from Dnmt3b1 mice (2.9 ± 0.6, n = 10) was significantly higher than Igf2 expression in control mice (1.3 ± 0.4, n = 11), P < 0.03 Mann Whitney U-test. (D) Quantitation of Sfrp expression in colon tumors normalized to β-actin expression using real-time PCR. To facilitate comparisons in one graph, measurements of each Sfrp RNA were normalized to the average value of the respective control group. The expression of Sfrp2, Sfrp4, and Sfrp5 was significantly lower in tumors derived from Dnmt3b1 mice (Sfrp2, P < 0.002; control, 1 ± 0.3, n = 10; Dnmt3b1, 0.35 ± 0.2, n = 10; Sfrp4, P < 0.05; control, 1 ± 0.3, n = 9; Dnmt3b1: 0.26 ± 0.15, n = 10; Sfrp5, P < 0.003; control, 1 ± 0.3, n = 9; Dnmt3b1: 0.29 ± 0.1, n = 10, Mann-Whitney U-test). Values represent mean ± SE.