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. 2007 Dec 1;21(23):3110–3122. doi: 10.1101/gad.1594007

Figure 5.

Figure 5.

Dnmt3b1 overexpression causes biallelic methylation of the H19 DMR but does not affect methylation of the Snrpn DMR1. (A) Bisulfite sequencing analysis of the H19 DMR of tumor DNA and colon mucosa DNA derived from control (right columns) and Dnmt3b1-expressing (left columns) mice (five tumors each and three colon mucosa samples each). The region analyzed covers 16 CpG sites. Each horizontal line represents one individual sequence; each block of sequences represents data from one sample. (Black boxes) Methylated CpGs; (white boxes) unmethylated CpGs. For each sample, the biallelic or monoallelic status of the methylation pattern was assumed from the ratio of methylated and unmethylated alleles. Four out of five Dnmt3b1-expressing tumors showed clearly increased H19 DMR methylation, whereas all control tumors showed mostly monoallelic methylation. (B) Bisulfite sequencing analysis of the Snrpn DMR1 of tumor DNA and colon mucosa DNA derived from control (right column) and Dnmt3b1-expressing (left column) mice (five tumors each and three colon mucosa samples each). Sixteen CpG sites were analyzed. Only one Dnmt3b1-expressing tumor showed clear hypermethylation at this site. A second tumor was possibly hypermethylated; however, the number of sequences is low. All colon mucosa samples show monoallelic methylation.