Figure 1.
Expression of lsiRNAs in Arabidopsis. (A–E) Expression of AtlsiRNA1–5 at 15 hpi of Pst DC3000 carrying an empty vector (EV) or the effector gene avrRpt2, avrRpm1, avrRps4, or avrPphB. AtlsiRNA-2 is also detected in the leaves sprayed with 2 × 105 spores per milliliter Alternaria brassicicola and harvested 2 and 4 dpi. (F) Expression of AtlsiRNA-6 in cell suspension culture. A diagram of genome position for each lsiRNA (black arrowhead) is shown below each panel. The arrowheads above the gene indicate the lsiRNAs with the same sequence as the gene, whereas the arrowheads below the gene indicate the lsiRNAs with the complementary sequence to the gene. Seventy-five micrograms of low-molecular-weight (LMW) RNA were used in each sample. The oligonucleotide probes complementary to the lsiRNA sequences were used for A–D (Supplementary Table S1). Ribo-probes synthesized for the overlapping region complementary to the sense genes At4g01700 and At5g58120 are employed for E and F. (The bars show the probe region.) U6 RNA was used as a control for measuring the relative abundance (RA) (shown below). Ethidium bromide-stained tRNA is shown as a loading control.