Skip to main content
. 2007 Dec 1;21(23):3149–3162. doi: 10.1101/gad.439507

Figure 7.

Figure 7.

ORF3* expression is stimulated by supplementation of translation assays with eIF3. (A) CrPV-2/3* mRNA (100 μg/mL) was translated in the presence of varying concentrations of supplementary eIF3. The yields of the ORF3* and ORF2 products were determined by densitometry of the autoradiographs, and the molar yield ratio is plotted against the concentration of added eIF3. (B) Selected mRNAs from the deletion series shown in Figure 3C (but in the background of the construct with the CrPV IRES) and the −64/62 point mutation in the same background were translated (at 80 μg/mL) in the presence or absence of 250 nM supplementary eIF3. (C) Uncapped transcripts of full-length pCSFV-2/3*, or (in the same background) the −64/62, −54/52, and −21/19 point mutants were translated in the absence or presence of 250 nM supplementary eIF3. (D) Translation of CSFV-2/3* mRNA (80 μg/mL) in eIF3-depleted RRL (dep), with or without supplementation with 150 nM eIF3. Numbers below each lane show the relative ORF3*/ORF2 product yield ratio (i.e., reinitiation efficiency) determined and defined as in Figure 4.