Table 2.

Distance metrics associated with probe set sensitivity to F/T RNA degradation. Probe sets were classified as invariant (INV) to degradation or as decreasing in signal (DEC) in response to degradation. DEC probe sets were further divided into two classes based on probe set location relative to the corresponding reference sequence termini.

Distance metric	INV	DEC	DEC (5'-3' < 1000)	DEC (5'-3' > 1000)
5'-3' distance	638 ± 386	1381 ± 665a	646 ± 171	1637 ± 576b
3'-3' distance	211 ± 388	973 ± 653a	289 ± 228	1212 ± 579b
5'-5' distance	1072 ± 783	904 ± 764	1250 ± 874	784 ± 689
RefSeq length	1711 ± 845	2285 ± 901a	1896 ± 830	2420 ± 891b
TargetSeq length	479 ± 72	451 ± 109	406 ± 111c	467 ± 105
TargetSeq/RefSeq	0.37 ± 0.21	0.22 ± 0.10a	0.25 ± 0.14c	0.21 ± 0.08b
Avg log2 RIN 9.5 signal	11.5 ± 1.3	10.0 ± 1.7a	10.0 ± 1.7c	10.1 ± 1.8b
Count	61	89	23	66

^aP < 0.0001 in unpaired two-tailed t-test comparisons of INV and DEC sets

^bP < 0.001 in a Tukey's post-test comparison of a one-way ANOVA

^cP < 0.01 in a Tukey's post-test comparison of a one-way ANOVA