Table 1.

Nucleotide Binding and Hydrolysis by Full-Length gpA, gpA-ΔN179 and gpA-ΔN179,K497D

Nucleotide	gpA-FL	gpA-ΔN179	gpA-ΔN179,K497D
aATP	KD,app= 0.8 ± 0.1 mM	KD,app= 1.7 ± 0.6 mM	KD,app= 2.5 ± 0.6 mM
aADP	KD,app= 1.2 ± 0.2 mM	KD,app= 63 ± 10 μM	KD,app= 192 ± 26 μM
AMP	bN.C.	bN.C.	bN.C.
Single-Turnover ATPase Activity	ck1= 0.706 ± 0.089 min−1 k2= 0.017 ± 0.025 min−1	dkobs= 0.086 ± 0.008 min−1	dkobs= 0.0072 ± 0.0004 min−1

^aThe fluorescence-monitored titration data presented in Figure 3 were quantified as described in Materials and Methods.

^bNo Change.

^cData taken from⁴².

^dThe kinetic data presented in Figure 4C were quantified as described in Materials and Methods.